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载脂蛋白B-48(ApoB-48)检测

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ApoB-48(Apolipoprotein B-48)是小肠来源的乳糜微粒(CM)中的载脂蛋白之一,是存在于乳糜微粒残粒中的特异性结构蛋白。

由于CM负责将食物来源的外源性脂质运输到肝脏和外周组织,因此ApoB-48-被认为是观察进食后外源性脂质运输的合适指标。FUJIFILM Wako可提供载脂蛋白B-48(ApoB-48)ELISA试剂盒。



◆载脂蛋白B-48(ApoB-48)应用


1. 通过检测同一样品中的LDL-胆固醇、HDL-胆固醇和B-48,可以外源性胆固醇、内源性胆固醇的变化。2

2. 空腹ApoB-48或可作为餐后高脂血症的标志物。9)

3. 空腹ApoB-48可用于研究患动脉粥样硬化疾病的风险。

4. ApoB-48还可用于评估CM残粒(CM Remnant),CM残粒为PAD(外周动脉疾病)、心血管疾病、血管内皮损伤和心血管系统的动脉粥样硬化的

 原因之一。


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ApoB-48 ELISA试剂盒  使用数据


ApoB-48是乳糜微粒的特异性结构蛋白,可作为观察进食等来源的外源性脂质运输。



进食后的甘油三酯(TG)与ApoB-48、总ApoB关系


参考Nakano,T. et al. Ann Clin Biochem, Vol.48, p57-64, Jan 2011.作图5)


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健康的6名男性与6名女性,39~60岁(平均52岁),空腹12 h后,口服OFTT奶油(17 g/m²)

结果显示,甘油三酯ApoB不相关,但ApoB-48相关



单独给药Fluvastatin和Fluvastatin+Omega-3 fatty acid中的ApoB-48和ApoB-100对比


参考Omega 3 fatty acids induce a markerd reduction of apolipoprotein B48 when added to fluvastatin in patients with type 2 diabetes and mixed hyperlipidemia Valdivielso,P. et al.Cardiovasc Diabetol, 8;8:1, Jan 2009.作图1


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2型糖尿病+高脂血症患者,Fluvastatin 80 mg组,Fluvastatin 80 mg+Omega-3 fatty acid 4g组,空腹12 h后抽血。

结果显示,ApoB-48作为指标更易评估,对比更明显



ApoB-48 ELISA试剂盒  使用数据


将本产品应用于高脂血症、动脉粥样硬化等的转化研究。



Ezetimibe给药引发的血液中ApoB-48、甘油三酯和胆固醇的变化


参考Kinoshita,M. et al.Exp.Anim. Vol.59(4), p459-467,2010.作图3)


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兔:NZW、♂、10周龄、体重2.5~3.0 kg、投喂高脂肪饲料

【Day1】投喂高脂肪饲料      【Day8】分为2组

● 对照组 n=3:3 mL生理盐水

● Ezetimibe给药 n=3:口服给药0.6 mg/kg 体重/每日3 mL生理盐水

   SE、* p<0.05、** p<0.01

结果显示,没有观察到TG和Cho的明显减少,但ApoB-48明显减少。



产品列表


产品编号

产品名称

规格

637-10641

Human ApoB-48 ELISA Kit

人血清载脂蛋白B-48(ApoB-48)ELISA试剂盒

96 tests

628-04901

Rabbit Apo B-48 ELISA Kit

兔血清载脂蛋白B-48(ApoB-48)ELISA试剂盒

96 tests



◆参考文献


1.

Omega 3 fatty acids induce a markerd reduction of apolipoprotein B48 when added to fluvastatin in patients with type 2 diabetes and mixed hyperlipidemia Valdivielso,P.,Rioja,J.,Garcia-Arias,C.,Sanchez-Chaparro,MA.,Gonzalez-Santos,P. Cardiovasc Diabetol, 8;8:1, Jan 2009 Otokozawa,S.,Ai,M., Diffenderfer,MR., Asztalos,BF.,Tanaka,A., Lamon-Fava,S., Schaefer,EJ. Metabolism Clinical and Experimental, Vol.58(11), p1536-1542, Nov 2009.

2.

Fasting and postprandial apolipoprotein B-48 levels in healthy,obese,and hyperlipidemic subjects

3.

Determination of Immuno-Reactive Rabbit Apolipoprotein B-48 in Serum by ELISA. Makoto, Kinoshita., Teruhiko, Matsushima., Yamato, Mashimo, Masaaki, Kojima., Megumi, Kigure. and Tamio, Teramoto. Experimental Animals, 59(4),459-467,2010

4.

Postprandial apolipoprotein B48 is associated with asymptomatic peripheral arterial disease: A study in patients with type 2 diabetes and controls Valdivielso, P., Puerta, S., Rioja, J., Alonso, I., Ariza, M. J., Sanchez-Chaparro, M. A., Palacios, R., Gonzalez-Santos, P. Clinica Chimica Acta, Vol.411(5-6), p433-437, Mar 2010.

5.

Clinica Chimica Acta, Vol.411(5-6), p433-437, Mar 2010. 5: Particle Size of ApoB-48 Carrying Lipoproteins in Remnant Lipoproteins Isolated from Postprandial Plasma Nakano, T., Tanaka, A., Okazaki, M., Tokita, Y., Nagamine, T. and Nakajima, K. Ann Clin Biochem, Vol.48, p57-64, Jan 2011.

6.

Postprandial lipoprotein metabolism: VLDL vs chylomicrons Nakajima K, Nakano T, Tokita Y, Nagamine T, Inazu A, Kobayashi J, Mabuchi H, Stanhope KL, Havel PJ, Okazaki M, Ai M, Tanaka A. Clin Chim Acta. Vol.412(15-16), p1306-18, Jul 2011.

7.

The characteristics of remnantlipoproteins in the fasting andpostprandialplasma the fasting andpostprandialplasma Nakajima K, Nakano T, Tokita Y, Nagamine T, Yatsuzuka S, Shimomura Y, Tanaka A, Sumino H, Nara M, Machida T, Murakami M. Clinica Chimica Acta, Vol.413(13-14), p1077-86, Jul 2012.

8.

Fasting apolipoprotein B48 is associated with asymptomatic peripheral arterial disease in type 2 diabetic subjects: A case-control study Lapice E, Cipriano P, Patti L, Romano G, Vaccaro O, Rivellese A A. Atherosclerosis, Vol.223(2), p504-506, Aug 2012.

9.

Effect of combination therapy with Fenofibrate and Simvastatin on postprandial lipemia in the ACCORD Lipid Trial Gissette Reyes-Soffer, et al. Diabetes Care, Vol.36, Feb 2013.

10.

Apolipoprotein B-48: A Unique Marker of Chylomicron Metabolism  Nakajima K, et al. Advances in Clinical Chemistry, Vol.64, 2014.



◆相关产品


LBIS® 人血清载脂蛋白B-48(ApoB-48)ELISA试剂盒

LBIS® 兔血清载脂蛋白B-48(ApoB-48)ELISA试剂盒



※ 本页面产品仅供研究用,研究以外不可使用。


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